Goldberg 2.5.1



  • Optics Letters
  • Vol. 42,
  • Issue 5,
  • pp. 995-998
  • (2017)
  • https://doi.org/10.1364/OL.42.000995
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    William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, and Jerome Mertz, 'Extended depth-of-field microscopy with a high-speed deformable mirror,' Opt. Lett. 42, 995-998 (2017)
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Abstract

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

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© 2017 Optical Society of America

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2.5.1

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